Czapek Dox Agar

Specifications

Sucrose 30.000 G/L
Sodium nitrate 2.000 G/L
Dipotassium phosphate 1.000 G/L
Magnesium sulfate 0.500 G/L
Potassium chloride 0.500 G/L
Ferrous sulfate 0.010 G/L
Agar 15.000 G/L
Final pH (at 25°C) 7.3 ñ 0.2

Directions :
Suspend 49.01 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium
completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C.
Mix well and pour into sterile Petri plates.
Principle :
Sucrose is the sole carbon source, and Sodium Nitrate is the sole nitrogen source in Czapek – Dox
Agar. Dipotassium Phosphate is the buffering agent, and Potassium Chloride contains essential
ions. Magnesium Sulfate and Ferrous Sulfate serves as sources of essential ions. Agar is the
solidifying agent in Czapek Dox Agar.