Cetrimide Agar Base

Specifications

Ingredients : gms/lit.
Pancreatic Digest of Gelatin 20.00
Magnesium Chloride 1.40
Potassium Sulphate 10.00
Cetrimide 0.30
Agar 15.00
Final pH (at 25°C) : 7.2 + 0.2

Directions :
Suspend 46.7 grams in 1000 ml distilled water containing 10 ml of glycerol. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.Cool to 45- 50°C. If desired, rehydrated contents of 1 vial of Nalidixic Selective Supplement (BF035) may be added aseptically to 1000 ml medium. Mix well and pour into sterile Petri plates.

Principle :
Pancreatic Digest of Gelatin provides the nitrogen, vitamins and amino acids in Cetrimide Agar Base. Magnesium Chloride and Potassium Sulfate enhance the production of pyocyanin and fluorescein. Cetrimide (cetyltrimethylammonium bromide) is the selective agent. Cetrimide acts as a quaternary ammonium cationic detergent causing nitrogen and phosphorous to be released from bacterial cells other than P. aeruginosa. Agar is the solidifying agent. Cetrimide agar base is supplement with 1%
Glycerol as a source of carbon.