Antibiotic Assay Medium No. 10 (Polymyxin Seed Agar)

Casein enzymic hydrolysate g/L 17.0
Papaic digest of soybean meal g/L 3.0
Sodium chloride g/L 5.0
Dextrose g/L 2.5
Dipotassium phosphate g/L 2.5
Agar g/L 12.0
Final pH (at 25°C) 7.2 +/- 0.2