Anaerobic Agar (Brewer)

Specifications

Proteose peptone 10.000 G/L
Casein enzymic hydrolysate 5.000 G/L
Yeast exctract 5.000 G/L
Dextrose 10.000 G/L
Sodium chloride 5.000 G/L
Sodium thioglycollate 2.000 G/L
Sodium formaldehyde sulfoxylate 1.000 G/L
Resazurin 0.002 G/L
Agar 15.000 G/L
Final pH (at 25°C) 7.2 ñ 0.2 G/L
pH range 7.00 - 7.40
Reaction reaction of 3.5% w/v aqueous
solution at 25°C pH 7.2 ñ 7.40


Directions :
Suspend 53 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize
by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile
Petri plates.
Principle :
Proteose peptone, casein enzymichydrolysate, yeast extract provides nitrogen, vitamin and amino acids.
Dextrose is a carbohydrate source. This medium contains sodium thioglycollate and sodium formaldehyde
sulphoxylate that provide adequate anaerobiosis, which is indicated by resazurin present in the medium.
Resazurin imparts pink colour to the medium in presence of oxygen. Brewer devised this medium for use
with Brewer anaerobic cover to permit surface growth of anaerobes and microaerophiles on agar without
the use of anaerobic jar. For best results, use porous tops on the plates containing the medium during
solidification to obtain a dry surface. After inoculation of the medium, cover with Brewer anaerobic petri
plate cover. The sealing ring inside the cover should make a perfect contact with the medium and must
not be broken before the end of the incubation period.