ALKALINE SALINE PEPTONE WATER

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Specifications

Ingredients g/L
Peptone 10.00
Sodium chloride 10.00
Final pH (at 25°C) : 8.4 + 0.2

Direction
Suspend 20.0 grams in 1000 ml distilled water. Heat if necessary, to dissolve the medium
completely. Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Principle
Peptone provides nitrogen and carbon source, long chain amino acids, vitamins and other essential nutrients. Sodium chloride maintains osmotic equilibrium

QC Tests
(I) Dehydrated Medium
Colour : Cream to light yellow
Appearance : Homogeneous Free Flowing powder
(II) Rehydrated medium
pH (post autoclaving/heating) : 8.4 ± 0.2
Colour (post autoclaving/heating) : Pale Yellow to light yellow
Clarity (post autoclaving/heating) : Clear

(III) Q.C. Test Microbiological
Cultural characteristics observed after 18 –24 hrs at 35-37°C.
MICROORGANISM (ATCC ) GROWTH
Vibrio parahaemolyticus (17802 ) Luxuriant
Vibrio cholerae (15748 ) Luxuriant

Use
For enrichment of Vibrio species.

Storage
Dehydrated medium-below30°C Prepared medium- Between 2 to 8°C.